The authors also thank the Lee Foundation for its support, Huang Dachuan and Waraporn Chan-on for proofreading the manuscript, and Sabrina Noyes for assistance in manuscript submission. *, P < 0.05 compared with vehicle control (vehicle). Effects of CP-690550 on NKTCL cell lines. This study was approved by the SingHealth Centralized Institutional Review Board (CIRB), study number 2004/407/B. Mr Gan first joined ComfortDelGro Corporation Limited as Group Corporate Planning Officer in February 2006. The next step uses a GATK Unified Genotyper that does the consensus calling to identify SNVs. Lim, D. Tan, C. Goh, C.C.Y. Teh, S.T. Lee, I. Cutcutache, W. Yu, C.C.Y. As such, it is possible that the number of homozygous tumors reported is actually an underestimate and that this value should be regarded as a lower limit. Teh, S.T. DNA of frozen tissue and paired blood samples was isolated using a DNeasy blood and tissue mini kit and a QIAmp DNA blood midi kit (Qiagen), respectively, according to the manufacturer’s instruction. 4B). View Gan Chin Kiat’s profile on LinkedIn, the world’s largest professional community. Lim, C. Goh, W. Yu, C.C.Y. Choon Kiat Chen Solutions Director , China Mobile International Choon Kiat has more than 25 years in the Telco/ICT industry since attaining his degree in Computing Science(1994) and MBA(2000). Contact Information. Koo, S.Y. Further validation of the prevalence of JAK3 mutations was determined by Sanger sequencing and high-resolution melt (HRM) analysis in an additional 61 cases. These results indicate that the JAK3-activating mutations are gain-of-function alleles and contribute to the constitutive activity of the JAK/STAT pathway in an IL-2–independent manner. "Every month or so we will be working with our public transport operators to have more themed trains in the next year," he said. Lee, L.C. Artnexus Design is the appointed creative agency for the NDP 2013 publicity production. The relative p-JAK3, p-STAT5, JAK3, and STAT5 levels in these cells were detected by Western blotting (top), and proliferation assays using these cells were conducted for 48 hours with or without IL-2 (bottom). Data were analyzed by paired t test, and values significantly different from control are shown as *, P< 0.05; **, P< 0.005; ***, P< 0.001. ©2012 AACR. We conducted whole-exome sequencing and identified Janus kinase 3 ( JAK3 ) somatic–activating mutations (A572V and A573V) in 2 of 4 patients with NKTCLs. Yap, K.A. The report said Gan, 61, was taking responsibility for an “inappropriate relationship”. For FFPE samples, genomic DNA was extracted from one or two 10-μm slices from each sample by removal of paraffin followed by proteinase K digestion according to standard procedures. He is also on the board of 11 other companies. Lim, Study supervision: S. Rozen, B.T. Ong, P. Tan, B.T. Yuen Kin Pheng is Professional at Tasek Corp. Bhd. Moreover, treatment of both JAK3-mutant and wild-type NKTCL cell lines with a novel pan-JAK inhibitor, CP-690550, resulted in dose-dependent reduction of phosphorylated STAT5, reduced cell viability, and increased apoptosis. He currently oversees The GBN Mentoring Program together with Seow Kiat Wang.. Gan Chimeg. These data are compelling and suggest a potential target for this otherwise fatal disease. FFPE tissue blocks from 61 patients with NKTCLs were procured for mutation analysis. This article is highlighted in the In This Issue feature, p. 569. The JAK3A572V and JAK3A573V mutations were located at exon 12, in the Janus homology domain 2 (JH2) that negatively regulates the Janus homology domain 1 (JH1) kinase activity (Fig. The coding exons of JAK3 were fully sequenced in these 3 cell lines, and we confirmed that only NK-S1 harbored a homozygous JAK3A572V mutation. In this study, we conducted whole-exome sequencing to identify somatic mutations in protein-coding genes of NKTCL tumors to shed light on their pathogenesis and to uncover potential new therapeutic targets, which are urgently needed. C, drug-induced apoptosis was evaluated by Annexin V-FITC (fluorescein isothiocyanate) staining, followed by flow cytometric analysis. 8). Land Transport Authority (LTA) Singapore is a entity based in 1 Hampshire Road, Singapore, 219428, Singapore, which employs 908 executives. Tan, T. Tang, S.L. Among these 4 members, JAK3 signaling is specifically related to T-cell development and proliferation (8) with loss-of-function mutations resulting in severe combined immunodeficiency characterized by the lack of T and NK cells (9). Lim, Supplied pathologic diagnosis for case series: L. Tan. Koo, S.Y. By continuing to use Gov.sg, you accept our use of cookies. 4C). Copyright © 2021 by the American Association for Cancer Research. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. Teh, S.T. 4A and B). HRM difference curves deviating from the wild-type curve were considered to be harboring a mutation. Teh, S.T. In accordance, transiently overexpressing a JAK3A572V in a JAK3 wild-type NKTCL cell line (KHYG-1) resulted in IL-2–independent cell proliferation and the activation of JAK/STAT signaling pathways. Conception and design: G.C. Ng, C.K. 3B). This study was funded by the National Medical Research Council of Singapore (NMRC/PPG/NCC/2011) as well as a grant from HSBC Trustee (Singapore) Limited as trustees of the Major John Long Trust Fund and the Chew Woon Poh Trust Fund. Sanger sequencing and HRM (25, 26) were used to confirm the JAK3 and JAK1 mutations identified and validate their prevalence in our NKTCL patient population. Choon Kiat Gan. Join Facebook to connect with Choon Kiat Gan and others you may know. Zhi Jiang Zang, Choon Kiat Ong, Ioana Cutcutache, Willie Yu, Shen Li Zhang, Dachuan Huang, Lian Dee Ler, Karl Dykema, Anna Gan, Jiong Tao, Siyu Lim, Yujing Liu, P. Andrew Futreal, Heike Grabsch, Kyle Furge, Liang Kee Goh, Steve Rozen, Bin Tean Teh, Patrick Tan Cancer Research, 1 January 2011 doi: 10.1158/0008-5472.CAN-10-1749 Choon Kiat Gan और अपने अन्य परिचितों से जुड़ने के लिए Facebook में शामिल करें. Others With a Similar Name. Thank you for sharing this Cancer Discovery article. K562 (CCL-234) was purchased from American Type Culture Collection and cultured in DMEM supplemented with 10% heat-inactivated FBS and 10% equine serum. In total, we found mutations in JAK3 in 23 of 65 (35.4%) cases (Supplementary Table S3) and for JAK1, besides the case with concomitant JAK1Y652D and JAK3A572V mutations described above, no additional mutations were identified. Cells were seeded at 2 × 104 cells/100 μL/well in 96-well plates and treated with or without CP-690550 (Selleck Chemical, S5001) at various concentrations before being subjected to MTS assay (Promega). This latter difference was not statistically significant (Supplementary Table S3). Poon, G.E. Currently, Choon Kiat Tan occupies the position of Group Financial Controller at SUTL Enterprise Ltd. Mr. Tan is also Member of Institute of Singapore Chartered Accountants. CP-690550, a novel pan-JAK inhibitor, has recently been shown to inhibit adult TCL/leukemia (ATLL) cells (22) and ATLL xenograft tumors and is currently in phase III trials for the treatment of rheumatoid arthritis (23). We do not retain these email addresses. We used Burrows Wheeler Aligner to align the sequence reads to the human reference genome NCBI built 37.1 (hg19) and then we ran SamTools to remove PCR duplicates. Recent data suggest that mutations resulting in persistent activation of JAK/STAT signaling are involved in the pathogenesis of T-cell acute lymphoblastic lymphoma/leukemia, cutaneous TCL, mantle cell lymphoma, acute megakaryoblastic leukemia, and myeloproliferative diseases (8, 10–16). Koo, S.Y. Treatment with CP-690550 resulted in reduced cell viability of NK-S1 and KHYG-1 cells but not in K562 cells. Several gene transcript annotation databases (CCDS, RefSeq, Ensembl, and UCSC) were used for transcript identification and for determining the amino acid change. Natural killer (NK) cells as a responder to interleukin 2 (IL 2). The molecular pathogenesis of natural killer/T-cell lymphoma (NKTCL) is not well understood. Sino Group is one of the leading property developers in Hong Kong. Lihat profil lengkap di LinkedIn dan terokai kenalan dan pekerjaan Choon kiat di syarikat yang serupa. Lihat profil Choon kiat Gan di LinkedIn, komuniti profesional yang terbesar di dunia. There has been little progress in basic science and clinical research in this subtype of lymphoma, which continues to constitute a major challenge in managing these patients as there is currently no accepted standard first-line treatment for NKTCLs. To detect single-nucleotide variants (SNV), we used a discovery pipeline based on the Genome Analyzer Toolkit (GATK). Acquisition of the data were conducted on FACSCalibur flow cytometer (BD Biosciences). Gan. 1). Tan, S.L. Gan Choon Beng has been a GBN Mentor since 2008 and has worked with many mentees in various areas of business. B, NK-S1 cell lysates were harvested for Western blotting, and the results showed that phosphorylation of JAK3 and STAT5 are IL-2–independent. The train, which will run till Dec 27, was launched at Ang Mo Kio station by Transport Minister Khaw Boon Wan, who rode it to Marina Bay station with nine students from Pathlight School. Poon, K. Tay, C.C.Y. Interleukin (IL)-2 is an essential cytokine required for the proliferation and activation of NK cells (6). Presently, Khai Choon Gan occupies the position of Non-Independent Non-Executive Chairman for HL Global Enterprises Ltd., Non-Executive Chairman of Beijing Fortune Hotel Co., Ltd. and Managing Director at Hong Leong International (Hong Kong) Ltd. Koo, S.Y. In parallel, these cells were harvested, and protein extracts were subjected to Western blotting with antibodies against phosphorylated JAK3 (p-JAK3), phosphorylated STAT5 (p-STAT5), JAK3, STAT5, or β-actin as a normalization control. Choon Kiat Gan is on Facebook. The average coverage of each base in the targeted regions was 111-fold; 84% of the bases were represented at least 20 times (Supplementary Table S1) and a total of 208 somatic mutations were identified in 201 genes (Supplementary Table S2). Among the patients with JAK3 mutations, there were 17 heterozygous JAK3A572V, 2 homozygous JAK3A572V, 2 heterozygous JAK3A573V, 1 homozygous JAK3A573V, and 1 heterozygous with both JAK3A572V and JAK3A573V mutations. Gan Choon Kiat, deputy director of the LTA's corporate transformation office, was quoted by Channel NewsAsia as saying that more themed trains will be rolled out in future. Gan Teng (Reno) Gan Teng. Mr Gan Juay Kiat was appointed Chief Executive Officer of SBS Transit Ltd on 1 March 2010. These results were further confirmed by high-resolution melt (HRM) analysis (Supplementary Fig. To decline cookies at any time, you may adjust your browser settings. Welcome to Singapore's directory of Specialist Doctors and Surgeons in private practice. By continuing to use Gov.sg, you accept our use of cookies. Choon kiat menyenaraikan 6 pekerjaan pada profil mereka. Lim, Administrative, technical, or material support (i.e., reporting or organizing data, constructing databases): S.L. In parallel, 50% of extra-nasal cases possessed JAK3 mutations and 31.7% of nasal cases had JAK3 mutations. We conducted whole-exome sequencing and identified Janus kinase 3 (JAK3) somatic–activating mutations (A572V and A573V) in 2 of 4 patients with NKTCLs. However, this phenomenon was not observed in the K562 cells in which STAT5 phosphorylation is dependent on BCR/ABL1 (8) and not JAK3 (Fig. To date, transforming ability of the activating mutations of JAK3 (such as P132T, L156P, R172Q, E183G, Q501H, M511I, A572V, A573V, R657Q, and V722I) has been previously validated in Ba/F3 cells (8, 12, 19, 20). Our findings have important implications for the management of patients with NKTCLs. ): G.C. Thus, it is conceivable that the JAK3 mutation may play an important role in the pathogenesis of NKTCLs. View Yuen Kin Pheng’s professional profile on Relationship Science, the database of decision makers. Tan, K. Tay, B.T. Whole-exome sequencing was successfully conducted on fresh-frozen NKTCLs and paired blood samples from 4 different patients. We are glad to play a part in our nation's 48th birthday celebration. A Christmas-themed MRT train has started plying the North-South and East-West Lines. Domain structure of JAK3 (bottom) and the positions of JAK3A572V(c.1715C>T) and JAK3A573V (c.1718C>T; top) identified through Sanger sequencing of NKTCL samples. Subsequently, he was also appointed the Chief Executive Officer and Director of ComfortDelGro Bus Pte Ltd. Transient overexpression of these 2 constructs in KHGY-1 cells was then generated using Effectene Transfection Reagent (Qiagen Inc.). The cycling and melting conditions were as follows: 1 cycle of 98°C for 2 minutes; 39 cycles of 98°C for 5 minutes; 58°C for 10 minutes; 1 cycle of 95°C for 30 minutes; and a melt from 72°C to 95°C increasing at 0.2°C/s. All 3 missense mutations were predicted by PolyPhen to be damaging (5). Earlier this month, SMRT announced that trains themed after the upcoming Star Wars VII: The Force Awakens movie will start operating from mid-December. Captured DNA libraries were sequenced with the Illumina GAIIx Genome Analyzer, yielding 150 (2 × 75) base pairs from the final library fragments. Gan Xa. The JAK family of tyrosine kinases comprises 4 members: JAK1, JAK2, JAK3, and TYK2. ©2012 American Association for Cancer Research. There is thus an urgent need to identify novel genetic aberrations and potential treatment targets in NKTCLs. Cogan Gan. Ong, S. Rozen, P. Tan, B.T. See the complete profile on LinkedIn and discover Ang Choon Kiat’s connections and jobs at similar companies. We also conducted Epstein-Barr virus–encoded RNA (EBER) testing on all cases. Importantly, NK-S1 cells treated with JAK3 siRNAs exhibited a significant reduction in cell proliferation and also decreased autophosphorylation of JAK3 and STAT5, compared with cells treated with control siRNAs (Fig. View the profiles of people named Kiat Gan. Our pipeline first recalibrates the base qualities and realigns the sequence reads around micro-indels. The single EBER-negative case was a cutaneous deposit taken from a patient with EBER+ nasal NKTCLs (Supplementary Table S3). Interestingly, several somatic heterozygous Janus kinase (JAK) mutations were found in 2 separate samples. Find contact's direct phone number, email address, work history, and more. Apart from 4 older cases that cannot be interpreted, all but one case were positive for EBER, regardless of JAK3 mutation status. Enter multiple addresses on separate lines or separate them with commas. Cells were harvested at indicated time intervals after IL-2 or CP-690550 treatment. Amino acid changes corresponding to SNVs were annotated according to the largest transcript of the gene. Cancer Discov; 2(7); 591–7. 2B). Furthermore, Annexin V staining revealed that the reduction of NK-S1 viability was due to an increase in cellular apoptosis (Fig. The molecular pathogenesis of natural killer/T-cell lymphoma (NKTCL) is not well understood. To further confirm the involvement of JAK/STAT signaling in the survival of NKTCLs, we next evaluated the effect of a pan-JAK inhibitor, CP-690550, in NK-S1, KHYG-1, and K562 cells. In contrast, JAK3 wild-type KHYG-1 cells were clearly IL-2–dependent (Fig. I. Proliferative response and establishment of cloned cells. Signals were visualized with enhanced chemiluminescence (ECL; Amersham). JAK1 and JAK3 mediate IL-2 receptor signaling through phosphorylation of STAT transcription factors (7). We compared our variants against the common polymorphisms present in dbSNP 131 and in the 1000 genomes databases to discard any common SNPs. However, compared with the more common B-cell lymphomas, very little is known about its molecular characteristics and pathogenesis. 4A) and cell viability in a dose-dependent fashion (Fig. IL-2–independent growth and constitutive JAK3 and STAT5 phosphorylation in a JAK3A572V-mutant NKTCL cell line. A total of 3 μg of genomic DNA extracted from each sample was used for exome sequencing. Significance: Gene mutations causing NKTCL have not been fully identified. One tumor harbored both JAK1Y652D and JAK3A572V mutations, whereas the other tumor harbored a JAK3A573V mutation. To determine the prevalence of JAK1 and JAK3 mutations in NKTCLs, we Sanger sequenced an additional 61 NKTCL formalin-fixed, paraffin-embedded (FFPE) cases. JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. Total proteins were extracted with lysis buffer, resolved by SDS-PAGE gels, and blotted onto a nitrocellulose membrane. Reciprocally, KHYG-1 cells transiently overexpressing a mutated JAK3 (JAK3A572V) cDNA showed IL-2–independent proliferation and autophosphorylation of JAK3 and STAT5 (Fig. View Ang Choon Kiat Amos’ profile on LinkedIn, the world's largest professional community. We use cookies to tailor your browsing experience. Commuters can also grab the Christmas tree-shaped cards hanging from the handrails to use as greeting cards. If you don’t like our faces, listen to our fortnightly podcast E-Junkies where we lepak one corner with famous people, Your daily good stuff - AsiaOne stories delivered straight to your inbox, AsiaOne Online Pte Ltd. Company Registration No. A, the NK-S1 cells harboring JAK3A572V were able to grow in an IL-2–independent manner. We use cookies to tailor your browsing experience. Ng, V. Rajasegaran, H.L. As expected, both the NK-S1 and KHYG-1 cells showed a reduction of phosphorylated STAT5 (Fig. Both NK-S1 and KHYG-1 cells showed a dose-dependent reduction in STAT5 phosphorylation. For HRM analysis, SsoFast EvaGreen Supermix (Bio-Rad, catalog number 172-5200) was used in the amplification of the sample amplicons using the JAK3 HRM primers at a final concentration of 600 nmol/L on a Bio-Rad CFX96 Real-Time PCR detection system in replicates. He is a practicing lawyer and is a Partner at Infinitus Law Corporation and Head of its Corporate, Commercial and Technology Department. Experiments were repeated at least 3 times.y. The diagnosis of NKTCL was made according to the 2008 World Health Organization classification of tumors of the hematopoietic and lymphoid tissues (24). Ng, S.T. Allen, W.S. Functional characterization of the JAK3 mutations support its involvement in cytokine-independent JAK/STAT constitutive activation leading to increased cell growth. The JAK/STAT pathway is a key component in normal hematopoiesis. Teh, S.T. Gan Choon Beng is a practicing lawyer and is the Senior Director at Infinitus Law Corporation and head of its Corporate, Commercial and Technology Department.He graduated from the University of Singapore (now known as the National University of Singapore) in 1977 with an LLB (Hons.) The men's 1000 metres competition at the 2010 Asian Games in Guangzhou was held on 18 November 2010 at the Zengcheng Dragon Boat Lake. NK-S1 (JAK3A572V homozygous mutant) and KHYG-1 (wild-type JAK3) cells were cultured with or without recombinant human IL-2 up to 7 days and followed by MTS assay. For Sanger sequencing, PCR was carried out with Platinum Taq Polymerase (Life Technologies, catalog number 10966-083) and cycled at 95°C for 10 minutes; 39 cycles of 95°C for 30 seconds; 60°C for 30 seconds, 72°C for 1 minute, and a final extension of 72°C for 10 minutes. Poon, W.S. The train is decked with yuletide artwork including Santa hats and reindeer antlers on the windows which serve as photo opportunities, Channel NewsAsia reported. Join Facebook to connect with Choon Kiat Gan and others you may know. To decline cookies at any time, you may adjust your browser settings. eISSN: 2159-8290 All variants retained following this step were considered to be novel. These are some of the notable cases which SPD officers handled in 2011: In PP v Abdul Razak Bin Hamid and PP v Tan Chye Guan Martin, the accused persons were both heinous child molesters, dubbed the “Letterbox Molester” and “Heartbeat Molester” respectively.They engaged in ruses to con children into physical contact and committed the offences over 20 years and 11 years respectively. We offer the most extensive selection of academic programmes in Singapore, collaborating with leading universities worldwide to provide our students with diverse opportunities for overseas exposure. Gan Ja. Known somatic mutations in NKTCLs, such as TP53, KRAS, and NRAS (4), identified by exome sequencing were further validated by Sanger sequencing in the same tumors (Supplementary Table S2). This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. The extent of drug-induced apoptosis was evaluated by Annexin V-FITC (BD Biosciences) staining. B, KHYG-1 cells were transiently transfected with wild-type JAK3 (JAK3 WT) or mutated JAK3 expression vectors (i.e., JAK3A572V). The resulting products were run on an ABI 3730 DNA analyzer. The presence of nonmalignant stroma (our samples contained at least 50% tumor content) or tumor subclones makes it difficult to assess whether these “heterozygous” tumors might actually represent a mixture of JAK3 homozygous–mutated cancer cells admixed with normal tissue. This is the first time that the Land Transport Authority (LTA) has introduced a festive-themed train. Despite multiagent chemotherapy and involved-field radiotherapy, the 5-year overall survival rate is approximately 9% for non-nasal NKTCLs and 42% for nasal NKTCLs (2, 3). Tan, L. Tan, S.C. Chong, K. Tay, M. Tao, R. Quek, S. Loong, K.-W. Yeoh, S.P. The JAK3, JAK1, JAK2, and TYK2 mutation status in these samples was confirmed by Sanger sequencing of all coding exons. They also indicated that targeting the JAK/STAT pathway in this disease is a potentially effective therapeutic approach that warrants further investigation. Further validation of the prevalence of JAK3 mutations was determined by Sanger sequencing and high-resolution melt … Lim, Development of methodology: G.C. Through exome sequencing, we identified activating mutations of JAK3 that may play a significant role in the pathogenesis of NKTCLs. View Choon Chia's business profile as Infrastructure & Operation Director, Systems (Rail Asset O&M) at Land Transport Authority. The wild-type JAK3 and JAK3A572V expression vectors were kindly provided by Dr. Brian Druker. View the profiles of people named Choon Kiat Gan. Ng, V. Rajasegaran, S. Rozen, B.T. Sequencing PCR was carried out with ABI BigDye Terminator v3.1 (Life Technologies, catalog number 4337457) and cycled at 96°C for 1 minute; 29 cycles of 96°C for 10 seconds; 50°C for 5 seconds, and 60°C for 4 minutes. The HRM curves were analyzed with the Bio-Rad Precision Melt Analysis Software. A, NK-S1 cells were treated with 100 nmol/L JAK3 siRNA (si-JAK3) or control siRNA (si-Ctrl) for 24 hours before being subjected to proliferation assays up to 72 hours (right). ISSN: 2159-8274, Sign In to Email Alerts with your Email Address. Choon Kiat Gan नाम के लोगों की प्रोफ़ाइल देखें. Between 8pm and 10pm from Dec 21-24, artists from the Band of Doodlers, the art collective behind the decorations, will be drawing portraits for commuters on the cards for free. The JAK1Y652D mutation was located in the JH2 domain as well. Ng, V. Rajasegaran, H.L. A research-intensive university with an entrepreneurial dimension, NUS is ranked consistently as one of the world's top universities. Lim, Acquisition of data (provided animals, acquired and managed patients, provided facilities, etc. D, phosphorylation of JAK3 and STAT5 in KHYG-1 cells were IL-2–dependent. Heng, A. Gan, S.T. See more people named Choonkiat Gan. With specialists from across all medical and surgical fields conveniently located islandwide, SingaporeDoc will help you find the right specialist. It accounts for up to half of all mature TCL cases in Asia (1). Gan Teng. Join Facebook to connect with Kiat Gan and others you may know. Besides hematologic neoplasia, nonhematologic cancers, including breast, stomach, and lung cancer, have also been found to harbor JAK3 mutations (17, 18). 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Through exome sequencing extracted from each sample was used for validation are listed in Supplementary Table S3 ) of..., whereas the other authors causing NKTCL have not been fully identified against the common polymorphisms present in 131! A GBN Mentor since 2008 and has worked with many mentees in various areas of business or! We also conducted Epstein-Barr virus–encoded RNA ( EBER ) testing on all cases अन्य परिचितों जुड़ने. Harboring a mutation Group is one of the Gene high-resolution melt ( HRM ) analysis ( Table. Up gan choon kiat lta half of all mature TCL cases in Asia ( 1 ) testing on all cases both JAK1Y652D JAK3A572V. Gan, 61, was taking responsibility for an “ inappropriate relationship ” its... Potential conflicts of interests were disclosed by the American Association for cancer Research Association cancer... Our nation 's 48th birthday celebration in contrast, JAK3 wild-type KHYG-1 cells were treated with respective... Law Corporation and Head of its Corporate, Commercial and Technology Department IL-2–independent manner domain as.! Countries and some parts of Latin America total proteins were extracted with lysis buffer resolved. Yang terbesar di dunia note: Supplementary data for this otherwise fatal disease cookies at any time, you know. The 1000 genomes databases to discard any common SNPs was evaluated by Annexin V-FITC ( BD ). 23 of 65 ( 35.4 % ) cases harbored JAK3 mutations in NKTCLs cancer Discov ; 2 ( )... It is conceivable that the reduction of phosphorylated STAT5 ( Fig surgical fields conveniently located islandwide, SingaporeDoc will you! % ) cases harbored JAK3 mutations and 31.7 % of nasal cases had JAK3 in... 72 hours B-cell lymphomas, very little is known about its molecular characteristics gan choon kiat lta.. Bd Biosciences ) staining in Supplementary Table S4 further analyzed virus–encoded RNA ( EBER ) testing on all.. With wild-type JAK3 ( JAK3A572V ) Tasek Corp. Bhd presence of activating JAK3.... Jak3-Activating mutations are gain-of-function alleles and contribute to the largest transcript of the data were conducted fresh-frozen. Deregulated JAK/STAT pathway in an IL-2–independent manner Beng has been a GBN Mentor since 2008 and has worked with mentees! The HRM curves were analyzed with the more common B-cell lymphomas, very is... Birthday celebration Kin Pheng is professional at Tasek Corp. Bhd acid changes corresponding to SNVs were according. The in this Issue feature, p. 569 connect with Choon Kiat Gan के... Mutations are gain-of-function alleles and contribute to the constitutive activity of the data conducted. Use of cookies the sequence reads around micro-indels लोगों की प्रोफ़ाइल देखें (. 2159-8290 ISSN: 2159-8274, Sign in to Email Alerts with your Email address genomic DNA extracted from each was. 4 different patients Kiat was appointed Chief Executive Officer of SBS Transit Ltd on 1 2010! By SDS-PAGE gels, and TYK2 you may adjust your browser settings of. Khgy-1 cells was then generated using Effectene Transfection Reagent ( Qiagen Inc. ) he is also on the Genome Toolkit! Interestingly, several somatic heterozygous Janus kinase ( JAK ) mutations were predicted by PolyPhen to be damaging 5! Discover Gan ’ s largest professional community Tao, R. Quek, S. Rozen, 569! Di dunia approach that warrants further investigation ) at Land Transport Authority, NK-S1 cell were. Involvement in cytokine-independent JAK/STAT constitutive activation leading to increased cell growth our variants against the common present.
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